Journal: The Journal of Biological Chemistry
Article Title: Pyroglutamate and O -Linked Glycan Determine Functional Production of Anti-IL17A and Anti-IL22 Peptide-Antibody Bispecific Genetic Fusions
doi: 10.1074/jbc.M112.417717
Figure Lengend Snippet: The peptide-antibody genetic fusion neutralizes the IL22 activity equivalent to parental anti-IL22 antibody while lacking anti-IL17A neutralizing activity. A, kinetic analysis with human IL22 using a Biacore 2000. B, IL22-stimulated release of GRO-α production in HT29 cells. C, table summary of kinetic analysis with human IL17A using a Biacore 2000. D, IL17A-stimulated release of GRO-α in BJ human foreskin fibroblast cells. Error bars, S.D.
Article Snippet: Bound GRO-α was detected with anti-human GRO-α biotin (catalog no. BAF275, R&D Systems) at 40 ng/ml, followed by streptavidin-HRP (catalog no. DY998, R&D Systems), followed by a 1:1 mixture of H 2 O 2 and tetramethylbenzidine substrate (catalog no. DY999, R&D Systems), followed by 2 n H 2 SO 4 .
Techniques: Activity Assay